Research Article: Phytochemical analysis of different extracts of leaves of Nicotiana tabacum L. of Cambodia 
Sokunvary Oeung, Voleak Nov, Huykhim Ung, Koemlin Roum, Voleak Yin, Samell Keo, Sin Chea 

Nicotiana tabacum L. has been employed in healing of various ailments such as gastrointestinal disorders, abdominal discomfort, constipation, urinary tract obstruction, dental pain and dermatitis. This study was conducted to investigate the phytochemical components of leaf extracts of Nicotiana tabacum L. Dried leaves of Nicotiana tabacum L. were subjected to the Ultrasound-Assisted Extraction (UAE) with distilled water, methanol, ethanol, ethyl acetate or chloroform, each extract of which was further applied to the phytochemical analysis via color tests. The extracting yields of leaves of Nicotiana tabacum L. with different solvents gave the values of 13.16% (aqueous extract), 14.20% (methanol extract), 4.88% (ethanol extract), 8.20% (ethyl acetate extract) and 3.63% (chloroform extract). The  
phytochemical screening revealed the presence of alkaloids, phenolic compounds, tannins, flavonoids, cardiac glycosides, steroids, terpenoids, essential oils, resins, saponins, quinones and polypeptides in different extracts of  
Nicotiana tabacum L. leaves. The current study provides the phytochemical information in term of authentication and standardization of leaves of Nicotiana tabacum L. cultivated in Cambodia.

Keywords: Nicotiana tabacum L.; ultrasound-assisted extraction; phytochemical