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Research Article: Bioassay guided isolation of anti-inflammatory principles from Prismatomeris tetrandra (Roxb.) K. Schum
Nor Hayati Abdullah, Ong Boo Kean, Mazura Md. Pisar, Ling Sui Kiong, Yasodha Sivasothy, Nor Azah Muhammad Ali, Norulaiman Yusoff, Nurhazwani Muhd. Hirmizi, Amira Rina Nurdiana Muhd. Sabarudin, Khalijah Awang

Abstract
The objective of this study is to investigate the anti-inflammatory constituents of Prismatomeris tetrandra (Roxb.) K. Schum through bioassay-guided fractionation procedures. The methanol extract of the leaves (MLE) displayed marked anti-inflammatory effects on TPA induced mouse ear oedema and hyaluronidase inhibitory assays as compared to the reference compound. The chloroform fraction from MLE (CF) showed the highest potency and was further subjected to bioassay guided fractionation which resulted in ursolic acid (1) being the compound responsible for the anti-inflammatory activity, with 103.18μM and 0.42 mg/ear IC50 inhibition towards hyaluronidase (at 100 μg/ml) and TPA-induced inflammation in mice (at 2mg/ear), respectively.

Keywords: Prismatomeris tetrandra, hyaluronidase, TPA induced mouse ear oedema, ursolic acid, bioassay guided isolation