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GC-MS Analysis and In Vivo Anti-Aging Activity of Indonesian Ficus carica L. Ethanolic Extract
Syamsudin Abdillah, Erlinnisa Nirmala

Abstract
Many researchers have conducted analysis of GC-MS and the biological activity of Ficus carica, however, there has not been research on the phytochemical components and anti-ageing activity of ethanol extracts originating from Indonesia. The objective of this study is to investigate GC-MS analysis and in vivo anti-ageing activities of these particular ethanolic extracts. Materials and Methods: The ethanolic extract was analyzed using GC-MS and tested for the anti-ageing effect of F. carica leaves on mice treated with D-galactose. D-galactose (100 mg/kgBW/day) was subcutaneously injected daily, for 42 days, to build an ageing model. The extract of F. carica leaves (50, 150, and 450 mg/kgBW/day) was administered daily for 42 days, beginning on the second day. Spectrophotometry was used to measure the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity. Results: both moderatedose and high-dose extracts of F. carica leaves enhance SOD, GSH-Px, and catalase, and reduce MDA content significantly (P <0.05). The results obtained revealed the presence of about 13 phyto-compounds, along with various potential bioactive compounds, which could be useful for both industrial and pharmaceutical purposes. Conclusion: This study confirms that the ethanolic extract of F. carica leaves and its mechanisms can be associated with increased antioxidant enzymes, such as SOD and GSH-Px, as well as decreased production of MDA, and enhanced scavenging free of radicals.

Keywords: Medicinal plants; Ficus carica; Moraceae; ageing